Downregulation of microRNA-34 induces cell proliferation and
By A Mystery Man Writer
Description
Malignant mesothelioma (MM) is an aggressive tumor with a dismal prognosis, and the molecular alterations involved in this disease remain unknown. We previously reported that microRNA-34s (miR-34s) are methylated and downregulated in MM and may play an important role in the carcinogenesis of MM. In this study, we downregulated miR-34s in human mesothelial cells to investigate the cellular effect of miR-34 knockdown. For the cell study, we used LP-9, a human mesothelial cell line, and three human primary-cultured mesothelial cell lines. RNA-based miR-34a, -34b and -34c inhibitors were transfected into these cells, and their effects on proliferation and invasion were evaluated. A scramble RNA oligonucleotide was used as a control. The protein expression status was estimated using western blotting. After miR-34 inhibitor transfection, miR-34a, -34b and -34c were downregulated in all the examined mesothelial cell lines. miR-34 inhibitor transfection significantly increased cell proliferation in all of the mesothelial cell lines, compared with the scramble control. The invasive ability also increased in the miR-34 inhibitor transfectants, compared with the scramble control, in the LP-9 cell line. Western blotting confirmed the upregulation of c-MET, phospho-c-MET, and bcl-2 proteins in LP-9 cells after miR-34 inhibitor transfection. In conclusion, our study showed that the downregulation of miR-34s induced an oncogenic phenotype in non-malignant mesothelial cells. The present study, together with the results of our previous report, strongly suggest that miR-34s play an important role in the early carcinogenic process involved in the transformation of human mesothelial cells to MM.
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Down-regulation of the tumor suppressor miR-34a contributes to head and neck cancer by up-regulating the MET oncogene and modulating tumor immune evasion, Journal of Experimental & Clinical Cancer Research
![Downregulation of microRNA-34 induces cell proliferation and](https://media.springernature.com/m685/springer-static/image/art%3A10.1038%2Faps.2015.153/MediaObjects/41401_2016_Article_BFaps2015153_Fig4_HTML.jpg)
Sirolimus induces apoptosis and reverses multidrug resistance in human osteosarcoma cells in vitro via increasing microRNA-34b expression
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Deciphering signaling pathway interplay via miRNAs in malignant pleural mesothelioma - ScienceDirect
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MicroRNA-34 family: a potential tumor suppressor and therapeutic candidate in cancer, Journal of Experimental & Clinical Cancer Research
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Anti‐hsa‐miR‐59 alleviates premature senescence associated with Hutchinson‐Gilford progeria syndrome in mice
![Downregulation of microRNA-34 induces cell proliferation and](https://www.laboratoryinvestigation.org/cms/asset/592f13e5-3081-4099-a313-f69a2a06f7f1/gr3.jpg)
Role of Stromal Fibroblast–Induced WNT7A Associated with Cancer Cell Migration Through the AKT/CLDN1 Signaling Axis in Oral Squamous Cell Carcinoma - Laboratory Investigation
![Downregulation of microRNA-34 induces cell proliferation and](https://media.springernature.com/m685/springer-static/image/art%3A10.1007%2Fs11356-021-12509-5/MediaObjects/11356_2021_12509_Fig1_HTML.png)
MicroRNA: a novel implication for damage and protection against ionizing radiation
![Downregulation of microRNA-34 induces cell proliferation and](https://ars.els-cdn.com/content/image/1-s2.0-S2468294220301118-gr3.jpg)
A comparison between the effects of over-expression of miRNA-16 and miRNA-34a on cell cycle progression of mesothelioma cell lines and on their cisplatin sensitivity - ScienceDirect
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MicroRNA-34a: the bad guy in age-related vascular diseases
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miR‐30d is related to asbestos exposure and inhibits migration and invasion in NCI‐H2452 cells - Ju - 2017 - FEBS Open Bio - Wiley Online Library
![Downregulation of microRNA-34 induces cell proliferation and](https://cdn.amegroups.cn/journals/pbpc/files/journals/2/articles/4365/public/4365-PB5-R1.png/w300)
MicroRNAs in mesothelioma: from tumour suppressors and biomarkers to therapeutic targets - Reid - Journal of Thoracic Disease
![Downregulation of microRNA-34 induces cell proliferation and](https://www.csbj.org/cms/asset/9ba72a38-6146-4d03-9777-508c02aa166b/ga1.jpg)
Meta-analysis of miR-34 target mRNAs using an integrative online application - Computational and Structural Biotechnology Journal
![Downregulation of microRNA-34 induces cell proliferation and](https://ars.els-cdn.com/content/image/1-s2.0-S2468294220301118-gr2.jpg)
A comparison between the effects of over-expression of miRNA-16 and miRNA-34a on cell cycle progression of mesothelioma cell lines and on their cisplatin sensitivity - ScienceDirect
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Frontiers Targeting the microRNA-34a as a Novel Therapeutic Strategy for Cardiovascular Diseases
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